Tracing of Peroxidase Activity in Humic Lake Water     

U. Münster  E. Heikkinen  K. Salonen  H. De Haan 《洁净——土壤、空气、水》1998,26(3):158-166

An enzyme assay was developed for studies on peroxidase activities in humic lake water. 3,4-Dimethoxybenzyl alcohol (veratryl alcohol, VeraOH) was used as tracer substrate, and peroxidase (EC 1.11.1.7) activity was measured by high-performance liquid chromatography. The chemical stability of VeraOH and its application as peroxidase substrate was tested under light and dark conditions, different hydrogen peroxide (H₂O₂) concentrations and humic matter contents. VeraOH was stable under low UV radiation at in situ conditions in lake water (<0.010...0.25 kJ m^–2 d^–1), laboratory conditions (<0.05...0.30 kJ m^–2 d^–1), and low (1...100 μM) H₂O₂ concentrations. However, peroxides oxidized VeraOH above 1...10 mM H₂O₂ concentration in sterile Millipore-Q and humic lake water. Dark incubations showed little VeraOH oxidation products. The developed peroxidase assay was tested in the growth medium of Phanerochaete chrysosporium and a bacteria isolate (P.M.D. 20.4.3.1) from mesohumic lake Pääjärvi. Peroxidase activities were also measured in natural microbial communities under standard laboratory and under in situ conditions in humic lake water. Incubation times of about 5 to 12 days were usually needed to record significant (P < 0.05) peroxidase activities, in lake waters. In situ peroxidase activities varied in pelagial surface water (0...0.5 m) on a seasonal scale between 74 nmol L^–1 h^–1 and 273 nmol L^–1 (mean: 176 nmol L^–1 h^–1) and within the water column between 110 nmol L^–1 h^–1 and 800 nmol L^–1 h^–1 (mean: 500 nmol L^–1 h^–1) in polyhumic lake Mekkojärvi.  相似文献   

BOOK REVIEWS     

《The Professional geographer》1986,38(1):105-130

Book Reviewed in this article: Unfairly Structured Cities . Blair Badcock Religion and Rural Revolt . Janos M. Bak and Gerhard Benecke The Industrial Geography of Canada . Anthony Blackbourn and Robert G. Putnam The Cuban-American Experience, Culture, Images, and Perspectives. Thomas D. Boswell and James R. Curtis Quaternary Paleoclimatology . R. S. Bradley Development and Crisis in Brazil, 1930–1983 . Luiz Bresser Pereiras Centres of Origin in Biogeography . John C. Briggs Analytical Urban Geography . Martin Cadwallader Geomorphology . Richard J. Chorley , Stanley A. Schumm, and David E. Sugden South Africa: The Impact of Past Geographies . A. J. Christopher The Geography of Underdevelopment. A Critical Survey . D. K. Forbes Health Care in Developing Countries . Wilbert M. Gesler Place to Grow Old: The Meaning of Environment in Old Age . Stephen M. Golant The Emerging Marine Economy of the Pacific . Chennat Gopalakrishnan An Introduction to Agricultural Geography . David B. Grigg The Soviet Union: A Geographical Study . G. Melvyn Howe Geographical Aspects of Health: Essays in Honour of Andrew Learmonth . N. D. Mc Glashan and J. R. Blunden Physical Geography: A Landscape Appreciation . Tom L. Mc Knight Mountain Experience. The Psychology and Sociology of Adventure. Richard G. Mitchell Jr . Systematic and Regional Biogeography. Stanley A. Morain The Economic Transformation of American Cities. T. J. Noyelle and T. M. Stanback , Jr . Normal Accidents: Living With High-Risk Technologies. Charles Perrow Vanishing Farmland: A Legal Solution for the States. Sarah E. Redfield Applied Methods of Regional Analysis: The Spatial Dimensions of Development Policy. Dennis A. Rondinelli Urbanization in Romania. A Geography of Social and Economic Change Since Independence. Per Ronnas Statistical Techniques in Geographical Analysis. Gareth Shaw and Dennis Wheeler The Granite Garden: Urban Nature and Human Design. Anne Whiston Spirn Political Geography: Recent Advances and Future Directions. Peter Taylor and John House Geographia y Medio Ambiente. Manuel Valenzuela -Rubío Nãgara and Commandery: Origins of the Southeast Asian Urban Traditions. Paul Wheatley The Common Fisheries Policy of the European Community. Mark Wise Geography and Gender. Women and Geography Study Group of the IBG Technological Hazards. D. J. Zeigler , J. H. Johnson , S. D. Brunn  相似文献   

Spurenelementgehalt der Saale – ein Vergleich der Analysenergebnisse aus den Jahren 1950 und 1996     

G. Proft  W. Schrn 《Chemie der Erde / Geochemistry》2008,68(2):221-233

Thirty four elements dissolved in water and 33 elements bound in particulate matter were analysed in the small river Saale (Thuringia, Germany, MQ=23 m³/s) in 1996 and the results were compared with those obtained in 1950 (44 elements). Monthly sampling was used to eliminate fluctuations caused by seasons and weather. Comparison of the element contents of a river over a 40-year-time span provides interesting insight into the anthropogenic change in the catchment area of the river with regard to „global change”.Without taking into consideration systematic errors, the analysed elements can be divided into three groups:

(a) Elements whose average annual analytical values in 1996 were lower by >50% than those in 1950: Al, As, Ba, Fe, Pb, Zn (in solution), and As, B, Cr, Li, Mn, Pb, Se, Sn, U (suspended).

(b) Elements whose average annual analytical values in 1996 are in the range of those in 1950, i.e., are within 50–150%: Ca, Co, Cr, F, K, Li, Mn, Na, Si, Sr, Ti, U (in solution), and Hg, Cr, Cu, Sc, Sr, Ti, Zn (suspended).

(c) Elements whose average annual analytical values in 1996 were higher by >150% than those in 1950: B, Cd, Mg, Ni, Rb, Sc (in solution), and Ba, Ni, P, Zr (suspended).

The increases in the element concentrations are not only caused by wastewater. Acid rain and fertilizer affect the pH and the electrolyte status of soils and cause mobilization of elements. This can be a reason for the increase in the alkalies and alkaline earth. For most elements are higher and lower values were found in 1996 and are only partly caused by systematic errors in the methods used in 1950. Taking into consideration of the natural fluctuations some element values 1996 equal or are lower (Tabelle 10 and 12). Because of the 100–200% RSD (12 samples per year), it is almost impossible to decide whether the deviations are due to analytical errors or to natural causes. The bound part of the elements is considered to be the suspended portion (seston=particulate matter) and, for one and the same element the suspended portion is equal or higher than the portion in solution. In the case of natural plant stock in the catchment area, the erosion is small. The increase in farming caused a higher soil erosion in the river. Storm precipitation causes short-term (1–2 h) peaks in the suspended load with values 2–3 orders of magnitude higher. In the case of monthly sampling, such peaks are unlikely to be detected. This produces values of suspended loads that tend to be too small. Increases of the bounded elements together with the elements in solution cause increases of eutrophication in the ocean.The contents of elements transported in solution and in particulate form in the river Saale are not equal to the element contents of the upper earth crust. Weathering and the fluviatil element transport cause a fractionation of the thallassophile (enriched in ocean) and therraphile elements (enriched in continents). Thallassophile elements are Mg, Ca, Sr, B, As, U, Sn, Cd, Zn, Se (transported in solution) and U, Cr, Li, B, Ba, Se, Mn, Cu, P, Sn, Cd, Rb, Pb, S and Zn (transported in seston) and therraphile elements are Cs, Co, Sc, Ni, Ti, Fe, Al, and K. As a result a fractionation in thallassophile and therraphile elements results and influences the geochemical cycles like magmatic differentiations.  相似文献   

零位稳定性实验研究   总被引：1，自引：0，他引：1  

刘小生  范俊智 《四川测绘》2001,24(3):131-132

本文用国产悬挂带、导流丝、逆变器同GAK－1陀螺经纬仪的原配件进行了对比实验研究，并进行了分析讨论。  相似文献   

Effects of eruption history and cooling rate on lava dome growth   总被引：1，自引：1，他引：0  

J. H. Fink  N. T. Bridges 《Bulletin of Volcanology》1995,57(4):229-239

To better understand the factors controlling the shapes of lava domes, laboratory simulations, measurements from active and prehistoric flows and dimensional analysis were used to explore how effusion history and cooling rate affect the final geometry of a dome. Fifty experiments were conducted in which a fixed volume of polyethylene glycol wax was injected into a tank of cold sucrose solution, either as one continuous event or as a series of shorter pulses separated by repose periods. When the wax cooling rates exceeded a critical minimum value, the dome aspect ratios (height/diameter) increased steadily with erupted volume over the course of a single experiment and the rate at which height increased with volume depended linearly on the time-averaged effusion rate. Thus the average effusion rate could be estimated from observations of how the dome shape changed with time. Our experimental results and dimensional analyses were compared with several groups of natural lava flows: the recently emplaced Mount St Helens and Soufrière domes, which had been carefully monitored while active; three sets of prehistoric rhyolite domes that varied in eruptive style and shape; and two sets of Holocene domes with similar shapes, but different compositions. Geometric measurements suggest that dome morphology can be directly correlated with effusion rate for domes of similar composition from the same locality, and that shape alone can be related to a dimensionless number comparing effusion rate and cooling rate. Extrapolation to the venusian pancake domes suggests that they formed from relatively viscous lavas extruded either episodically or at average effusion rates low enough to allow solidified surface crust to exert a dominating influence on the final morphology.  相似文献   

半干旱半湿润地区洪水预报模型适用性研究   总被引：1，自引：0，他引：1       下载免费PDF全文

刘郁  赵兰兰  马丁 《水文》2016,36(1):32-36

针对半干旱半湿润地区的流域特性和产汇流特点,比较了河北雨洪模型和新安江模型的模型原理和模型特性,以滦河支流老牛河流域为例,开展了洪水预报适用性研究。结果表明,河北雨洪模型的预报精度较高,更适用于半干旱半湿润地区的洪水预报。  相似文献   

厦门西港航道潮流和悬沙回淤实验研究   总被引：1，自引：0，他引：1  

徐啸 《台湾海峡》1996,15(2):103-112

通过潮汐水流试验和定床浑水悬沙回淤试验，探求厦门西港嵩（屿）－鼓（鼓浪屿）水道及猴屿以南浅段航道水流动力特点及泥沙回淤机制，研究各种工程方案对航道及港区沙回淤规律的影响，预测港区航道今后泥沙回淤趋势。  相似文献   

Suspended Particles: Their Role in Estuarine Biogeochemical Cycles   总被引：11，自引：0，他引：11  

A. Turner  G. E. Millward 《Estuarine, Coastal and Shelf Science》2002,55(6):857

Suspended particles are instrumental in controlling the reactivity, transport and biological impacts of substances in aquatic environments, and provide a crucial link for chemical constituents between the water column, bed sediment and food chain. This article reviews the role of suspended particles in the chemical and biological cycling of trace constituents (trace metals, organo-metallic compounds and hydrophobic organic micropollutants; HOMs) in estuaries, with particular emphasis on the effects of and changes to particle reactivity and composition.The partitioning (or distribution coefficient,K_D ) and bioavailability of chemical constituents, and assimilation efficiency (AE) of such by bivalve suspension feeders, are identified as key parameters requiring definition for accurate biogeochemical modelling, and the discussion centres around the determination of and controls on these parameters. Particle-water interactions encompass a variety of physical, biological, electrostatic and hydrophobic effects, and are largely dependent on the character and concentration of suspended particles and salinity. The salinity-dependence results from the competing and complexing effects of seawater ions for trace metals, and the compression of water in the presence of dissolved seawater ions and consequent salting out of neutral solute (HOMs, organo-metallic compounds and some trace metal complexes). The extent of biological solubilization of chemical constituents from suspended particles is dependent on the nature of chemical components of the gastro-intestinal environment and their interactions with ingested particles, and the physiological (e.g. gut passage time) and chemical (e.g. redox conditions and pH) constraints imposed on these interactions. Generally, chemicals that associate with fine, organic-rich particles (or, for some HOMs, fine inorganic particles), and desorb at pH 5–6 and/or complex with digestive enzymes or surfactants are most readily solubilized in the gut. The extent of assimilation of solubilized chemical is then determined by its ability to pass the gut lining and partition into cytosolic material.In practice, K_D and AE are determined experimentally by means of radiotracers added to contained suspensions or mesocosms, while operational measurement of bioavailability relies on in vitro chemical or biological (enzymatic) extraction of particles. What is lacking, however, and is identified as an ultimate goal of future research, is the ability to predict these parameters from theoretical principles and thermodynamic constants. Since many of the inherent interactions and mechanisms are controlled by particle composition and reactivity, a more immediate objective would be better characterization of the biogeochemical properties of suspended particles themselves. This includes chemical resolution of the bulk organic matter, definition of the abundance and synergistic effects of component sorbent phases, and determination of the effects of particle-seawater ion interactions on the reactivity of the particle surface.  相似文献   

The Effect of an Infaunal Suspension Feeding Bivalve Mercenaria mercenaria (L.) on Benthic Recruitment*     

Don Maurer 《Marine Ecology》1983,4(3):263-274

Abstract. Animal-animal interactions are recognized as critical in benthic invertebrate recruitment. The hypothesis tested was that benthic recruitment is more successful in low densities of infaunal suspension feeders than in high densities. Densities of young hard clams Mercenaria mercenaria (L.) ranging from 82.5 to 330m^-2 were placed in defaunated boxes of sand. The mean number of species, mean number of individuals, mean wet weight biomass, mean species richness and mean dominance index per sample were calculated per density of hard clams from May to October. The experiment did not support the hypothesis. It was concluded that feeding behaviour of dense populations of M. mercenaria did not preclude successful recruitment of other benthic species.  相似文献   

不定积分变换法对二度体磁异常的研究Ⅰ-水平圆柱体、直立薄板的不定积分变换     

张维冈  裴建新 《中国海洋大学学报(自然科学版)》2003,33(5):777-782

作者从二度磁性体的磁异常公式出发,推导出各异常的不定积分变换结果,并由变换结果绘制不定积分变换的磁异常参量图.本文选取2种规则形态磁性体,在取不同空间几何参数的条件下,讨论它们不定积分变换后参量图的曲线变化规律,并总结其异常特征,而后将其与积分变换前的参量图对比进行磁异常讨论和效果分析.该方法能反映一些重要的地质信息,将有助于实际的磁异常解释.  相似文献